SubtiBank SubtiBank
Version comparison:

2017-8-11 16:0:32025-05-04 11:29:14

_ec

locus

BSU39230

BSU_39230

geneLength

7002

7005

outlinks

bsu

BSU39230

BSU_39230

The protein

[SW|Domains]

cell wall binding domain as retention signal

C-terminal toxic domain with RNase activity (cleaves tRNAs) [Pubmed|23572593]

cell wall binding domain as retention signal

C-terminal toxic domain with RNase activity (cleaves tRNAs) [Pubmed|23572593]

Has two ligand-binding domains; the N-terminus has three 101 AA repeats which are responsible for cell wall binding; the C-terminus consists of two blocks of residues with a conserved motif repeated 31 times (according to UniProt)

References

11987133, 16672620, 18957862, 16306698, 9537385, 21821766, 23572593, 23199363, 25666134, 26923784, 28827522

11987133, 16672620, 18957862, 16306698, 9537385, 21821766, 23572593, 23199363, 25666134, 26923784, 28827522, 32419322

The protein

Protein family

RHS/WapA nuclease family (single member, according to UniProt)

The protein

Paralogous protein(s)

[[this]]

Biological materials

Mutant

BKE39230 (Δ[[gene|wapA]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCTCTCCTTTTGT, downstream forward: _UP4_TAATAAGGTTAAGCGAGGGG

BKK39230 (Δ[[gene|wapA]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCTCTCTCCTTTTGT, downstream forward: _UP4_TAATAAGGTTAAGCGAGGGG